Kinetic analysis of pre-ribosome structure in vivo

DMS modification in vivo and purification of newly synthesized rRNAs. (A) Schematic representation of the labeling and purification procedure, described in the text. (B) Northern analyses of newly synthesized rRNAs. One percent of total RNA and 100% of IP RNA were used for analysis. RNAs were separated on a 1.5% BPTE agarose gel and transferred to nylon membrane. Oligo probes that were used to monitor intermediates of rRNA processing and 18S rRNA are indicated.