CycC/Cdk8 function in snRNA 3′-end formation independent of Mediators 12/13 and are associated with Integrator subunits. (A) Western blot analysis of lysates from S2 cells treated with dsRNAs targeting members of the Drosophila Mediator Cdk8 module and transfected with the U7-GFP reporter. (B) Western blot analysis of lysates from HeLa cells treated with siRNA to CycC or CDK8 followed by transfection with a human version of the U7-GFP reporter. (C) Immunoprecipitations using αFlag-agarose to detect interactions between CycC/Cdk8 and Integrators 1 and 9. Input lanes represent 2% of input, and IP lanes represent 50% of immunoprecipitate. (D) Western blot analysis of immunoprecipitations using anti-IntS12 antibodies.
