An RNAi screen identifies additional members of the Drosophila Integrator complex and a requirement for cyclin C/Cdk8 in snRNA 3′-end formation

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FIGURE 4.
FIGURE 4.

Asunder and CG4785 biochemically associate with the fly Integrator complex. (A) Western blot analysis of Flag-tagged Asunder or CG4785 expression in stable S2 cell lines. (B) IF analysis of stable S2 cell lines expressing Flag-tagged Asunder or CG4785. (C) Western blot analysis of Flag immunoprecipitates from cell lines described in A. Input lanes represent 5% of input, and IP represents 50% of the immunoprecipitate. (D) Directed yeast two-hybrid using fly Integrator subunits and CG4785 demonstrating an interaction with IntS10.

This Article

  1. Published in Advance October 24, 2012, doi: 10.1261/rna.035725.112 RNA 18: 2148-2156

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