FIGURE 7.
Mutations that rescued hDicer binding or cleavage. (A) Gel shift assay of the wild-type pre-miR-892a (lanes 1,2) and its M1 mutant (lanes 3,4). Mutations in M1 are marked with a box. (B) Predicted secondary structures of pre-miR-718 and its M1 and M2 mutants. Mutations are marked with a box. The RNAs were treated with Dicer for 5, 15, and 40 min. Sizes of DNA markers (in nucleotides) are indicated at the left. (C) Gel shift assay. (Lanes 1,2) Wild-type pre-miR-718; (lanes 3,4) M1; (lanes 5,6) M2. Pre-miR-892a and pre-miR-718 and mutants were tested at least twice, and representative results are shown in A–C.
