FIGURE 5.
Impacts of the stem and terminal loop on hDicer function. (A) Predicted secondary structures of pre-miR-16, pre-miR-30, pre-miR-31, and their mutants. (B) hDicer digestion of the pre-miRNAs and mutants. Sizes of DNA markers (in nucleotides) are indicated in the left. (C) Gel shift analysis of the binding of hDicer to the indicated pre-miR-30 and mutants. (D) Comparison of the relative hDicer cleavage and binding efficiencies for the pre-miRNAs and mutants listed in A. For relative hDicer cleavage and binding efficiencies, the respective, wild-type pre-miRNAs are set as 100%.
