FIGURE 2.
Differential pre-miRNA cleavage by hDicer. (A) Examples of end-point analysis of pre-miRNA cleavage by hDicer. Pre-miRNAs were incubated with hDicer for ∼60 min. Sizes of DNA markers (in nucleotides) are indicated at the left. Shown are the representative results of at least three replicate experiments. (B) Increasing amounts (0.1–6 nM) of pre-miR-412, pre-miR-718, and pre-let-7a were incubated with 0.2 nM hDicer for 5 min. Sizes of DNA markers (in nucleotides) are indicated. (−) Without hDicer addition. Shown are the representative results of at least three independent experiments.
