FIGURE 1.
Characterization of hDicer preparation and pre-miRNA processing reactions. (A) Coomassie blue staining of a gel running the purified, recombinant hDicer protein. Protein markers (Bio-Rad) are shown in the left in kilodaltons. (B) hDicer processing of pre-let-7a, pre-miR-892a, and pre-miR-491 in a time course study. Reaction times are shown in the bottom in minutes. Positions of single-stranded DNA markers are indicated in nucleotides. (C) Quantification of hDicer cleavage ratios for the reactions shown in B. Averages and standard deviations in percentages are shown, based on three independent experiments.
