Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA endonucleases

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FIGURE 5.
FIGURE 5.

Functional analysis of Cas5d mutants. (A) Comparison of RNA endonucleolytic activity mediated by wild-type and Y27F Cas5d. (B) Comparison of RNA endonucleolytic activity mediated by wild-type and E23Q Cas5d. Denaturing PAGE analysis of 32P end-labeled repeat RNA incubated with 100 nM wild-type, Y27F, and E23Q Cas5d over a (left to right) 1-, 2-, 5-, 10-, 15-, 30-, 45-, 60-min time course.

This Article

  1. Published in Advance September 24, 2012, doi: 10.1261/rna.033100.112 RNA 18: 2020-2028

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