Effects of Cbc2 Y24A and NΔ42 mutations on SUS1 pre-mRNA splicing. RNAs isolated from cbc2Δ, CBC2, cbc2-Y24A, and cbc2-NΔ42 cells were reverse transcribed with an oligo(dT) primer, and the cDNAs were PCR-amplified with primers in the first and third exons of chromosomal SUS1 gene (depicted at the bottom). The PCR products were resolved by native agarose gel electrophoresis and visualized by staining with ethidium bromide. The right lane shows the product of PCR-amplification of genomic DNA with the SUS1 primers. The positions and sizes (bp) of linear duplex DNA markers are indicated on the right. The positions of the RT-PCR products of unspliced, partially spliced, and fully spliced SUS1 transcripts are indicated at left.
