Genetic interactions of hypomorphic mutations in the m⁷G cap-binding pocket of yeast nuclear cap binding complex: An essential role for Cbc2 in meiosis via splicing of MER3 pre-mRNA

The cap-binding pocket of CBC. Stereo view of the CBP20 subunit of the human CBC (from pdb 1H2T) highlighting the π-cation stack of the m⁷G cap nucleoside sandwiched between two conserved tyrosines and the hydrogen bonding contacts between conserved amino acid side chains and the m⁷G base, the cap ribose, and the bridging phosphates of the cap dinucleotide. The amino acids are labeled in the figure according to their residue numbers in the homologous Cbc2 subunit of yeast CBC.