A combination of visual and computer-based analysis reveals that the most significant RBP42-bound mRNAs encode proteins that function in energy production. Metabolism-associated genes with the highest enrichment values (top 10%) for RBP-42–bound RNA tags are depicted as dark green ovals; genes with above-background values are depicted as light green ovals. Enzymes are (1) hexokinase (HK); (2) glucose-6-phosphate isomerase (GPI); (3) phosphofructokinase (PFK); (4) aldolase; (5) triose-phosphate isomerase (TIM); (6) glycerol-3-phosphate dehydrogenase (G3PDH); (7) glycerol kinase-glycosomal (GKg); (8) glyceraldehyde-3-phosphate dehydrogenase (GAPDH); (9) phosphoglycerate kinase (cytosolic, PGKc, and glycosomal [PGKg]); (10) phosphoglycerate mutase (PGAM); (11) enolase (ENO); (12) pyruvate kinase (PYK); (13) phosphoenolpyruvate carboxykinase (PEPCK); (14) pyruvate phosphate dikinase (PPDK); (15) malate dehydrogenase-glycosomal (MDHg); (16) fumarase-cytosolic (FHc); (17) NADH-dependent fumarate reductase (FRDg); (18) adenylate kinase-glycosomal (AK), GIM5-glycosomal membrane protein (GIM5), and PEX11-glycosomal membrane protein (PEX11); (19) malic enzyme-cytoplasmic (MEc); (20) pyruvate dehydrogenase complex (PDH); (21) acetate:succinate CoA transferase (ASCT); (22) unknown enzyme; (23) succinyl CoA synthetase (SCoAS); (24) citrate synthase (CS); (25) aconitase (ACO); (26) isocitrate dehydrogenase (IDH); (27) 2-ketoglutarate dehydrogenase (ODGC); (28) succinate dehydrogenase (SDH); (29) fumarase-mitochondrial (FHm); (30) malate dehydrogenase-mitochondrial (MDHm); (31) proline dehydrogenase (PRODH); (32) pyrroline-5-carboxylate reductase (P5C reductase); (33) L-alanine aminotransferase (ALAT); (34) glutamate dehydrogenase (GDH); (35) glycerol-3-phosphate oxidase (FAD-G3PDH); (36) rotenone-insensitive NADH dehydrogenase (R–I); (37) alternative oxidase (AO); and (38) F0/F1-ATP synthase (β-, γ-subunits and subunit 9) in complexes I–IV of the respiratory chain (in complex I, subunit 20-kDa subunit is indicated; in complex II, no subunits are indicated; in complex III, the Rieske iron-sulfur protein is indicated; in complex IV, the cytochrome oxidase subunits IV, V, and VII–IX are indicated; cytochrome C [Cyto C]); (39) threonine-3-dehydrogenase (TDH); (40) 2-amino-3-ketobutyrate CoA ligase (ABK ligase); (41) transaldolase; and (42) transketolase.
