RBP42 is within the S100-precipitated fraction in the cellular cytoplasm. (A) Subcellular fractionation scheme is shown. (B) Immunoblotting of fractionated lysates identifies RBP42 as cytoplasmic and associated with the ribosome-containing subcellular fraction (S100 pellet). Protein detection was performed using the four antibodies listed on the left side of the panel. RPB1, the largest subunit of RNA polymerase II, is a nuclear marker; GRASP and BiP are cytoplasmic markers that fractionate with the S100 supernatant due to the early detergent step in the fractionation (Ho et al. 2006). (C) Immunofluorescent detection of fixed parasites shows cytoplasmic localization of RBP42. Four of 15 Z-stacks are shown. Z-stacks were collected to refine resolution.
