Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay

Isothermal titration microcalorimetric analyses of the interactions between eRF3a(64–94) and full-length PABPC1. (Upper panels) Trace of the calorimetric titration of 19 2-μL aliquots of eRF3a(64–94) into the cell containing PABPC1 in the presence (A) or absence (B) of A₁₀₀₀. (Lower panels) The integrated binding isotherm obtained from the experiment was fitted using a single-site model. Parameters obtained from the best fit (solid line) with error values calculated from the fittings are summarized in Table 3.