Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay

Overlay of the 20 structures of (A) PABC–eRF3a(64–82), PDB code 2rqg (BMRB code 10019), and (B) PABC–eRF3a(73–94), PDB code 2rgh (BMRB code 7033). (A,B) Backbone structures of the PABC residues 543–619 (green). Heavy atoms of eRF3a residues 65–80 and 75–88 are shown in A and B. Residue numbers for both termini are indicated. (C,D) Molecular surfaces of PABC, colored by the electrostatic potential, are shown with a ball-and-stick model of the bound eRF3a residues 65–80 (C) and 75–88 (D). The hydrophobic pockets on PABC (N- and C-pockets) are indicated by ovals.