Biological role of the two overlapping poly(A)-binding protein interacting motifs 2 (PAM2) of eukaryotic releasing factor eRF3 in mRNA decay

Isothermal titration microcalorimetric analyses of the interactions between eRF3a fragments and PABC. (A, upper panel) Trace of the calorimetric titration of 29 10-μL aliquots of eRF3a(51–102) into the cell containing PABC. (Lower panel) The integrated binding isotherm obtained from the experiment was fitted using a single-site model. The parameters obtained from the best fit (solid line) with the error values calculated from the fittings are summarized in Table 1. (B) Amino acid sequence alignment of the peptides used for the ITC analyses. PAM2-N (blue), PAM2-C (cyan), and their overlapping region (red). (Gray) Sequences derived from cloning artifacts.