Identification of novel antisense CUTs from the 2μ plasmid. (A) Distribution of RNA sequence reads from the 2μ plasmid. The chart shows the density of RNA-seq reads mapped to each strand (black or gray) of the B-form of the 2μ plasmid for strains with the indicated genotypes. (Block arrows below the chart) The positions and orientations of protein-encoding transcripts and line arrows indicate noncoding transcripts. The noncoding transcripts include two previously described RNAs overlapping RAF1 (the “1620b” RNA) and REP1 (the “1950b” RNA) and two novel transcripts 2μCUT1 and 2μCUT2 antisense to FLP1 and REP2, respectively. (B) Northern blot analysis of the levels of mRNA levels from strains with the indicated genotypes. Total RNA (20 μg) from each strain was separated by electrophoresis on a 1% agarose-formaldehyde gel, transferred to GeneScreen membrane (DuPont), and probed with strand-specific 5′ 32P-labeled oligonucleotides.
