Air proteins control differential TRAMP substrate specificity for nuclear RNA surveillance

Absence of Air1 causes the loss of 2μ plasmid and transcripts. (A) Graphical comparison of the relative log₂ fold change in the number of RNA-seq reads for the indicated 2μ plasmid transcripts between wild type (wt) and strains with the indicated genotypes. (B) Semiquantitative PCR analysis of 2μ plasmid DNA and chromosome VI levels for DNA from strains with the indicated genotypes. (C) Real-time qRT-PCR analysis of 2μ plasmid DNA levels from strains with the indicated genotypes. 2μ plasmid DNA levels are normalized to the amount of chromosome VI DNA determined by the same method.