Absence of Air2 decreases levels of mRNAs encoding proteins involved in glucose transport and metabolism while elevating levels of mRNAs involved in iron import. (A) Real-time qRT-PCR analysis of the indicated mRNAs after reverse transcription of poly(U)-Sepharose selected [poly(A)+] RNA with a mix of random primers and oligo(dT). (B) Same as in A, but with the indicated iron transport genes. (C) Northern blot analysis of the levels of FIT3, FIT2, and ACT1 mRNAs. Indicated strains were grown in YPD at 30°C, and 20 μg of total RNA was run on a 1% agarose gel and analyzed by Northern blotting for the indicated RNA species. (D) Differential growth phenotypes of 10-fold dilutions of cells with the indicated genotypes on SCD plates incubated with and without 4 mM FeCl2 for 3 d at 30°C.
