Absence of Air2 elevates levels of some snoRNAs and suppresses the 5FU toxicity of rrp6-Δ. (A,B) Real-time qPCR validation of Air2-specific snoRNAs targets. All histograms represent the fold change of the transcript compared to WT (RRP6) after normalization to ACT1 mRNA. Error bars represent standard deviations calculated from three independent reactions. (A) Real-time qRT-PCR analysis of snoRNAs after reverse transcription of poly(U)-Sepharose selected [poly(A)+] RNA with a mix of random primers and oligo-(dT). (B) Real-time RT-PCR of total isolated RNA after reverse transcription using a mix of random primers and oligo(dT). (C) Strains with the indicated genotypes and carrying the indicated yeast expression plasmids (YEp) were diluted to an OD600 of 0.5 and 10-fold dilutions were spotted onto SCD-URA plates with or without 2 μM 5FU. Plates were imaged after a 3-d incubation at 30°C.
