(A) Growth phenotypes of air1-Δ rrp6-Δ, air2-Δ rrp6-Δ, and air1-Δ air2-Δ strains after loss of complementing plasmids. The indicated strains were grown overnight in YPD media, resuspended at OD600 of 0.5 and 10-fold serial dilutions were plated on synthetic complete dextrose (SCD) or 5-FOA containing media. 5-FOA selects for cells that have lost the complementing YCp plasmids. The plates were incubated at 30°C and imaged after 2 d. The plate in the top panel was imaged a second time after 5 d of growth (far right panel). (B) Differential growth phenotypes of strains lacking Air1 or Air2. Cultures of strains with the indicated genotypes were diluted to an OD600 of 0.5, serially diluted 10-fold, and spotted onto SC glycerol (2%)–URA plates or SC dextrose (2%)–URA media with no addition, or with 10 mM caffeine, or 2 μM 5-fluorouracil and incubated for 3–5 d at 30°C. (B) Plating of strains with the indicated genotypes carrying yeast CEN plasmids as in (A) onto SC dextrose (2%)–URA or SC glycerol (2%)–URA plates.
