IRES-dependent translation of egr2 is induced under inflammatory conditions

Egr2-IRES activity is p38-MAPK- and IL-1β-dependent. (A) MCF7 cells were transfected with phpR-egr2-F. Forty-eight hours after transfection, cells were treated with Ctr, CM, or rIL-1β (50 ng/mL) for 4 h. IRES activity was calculated as described before and is given relative to Ctr. Data are presented as means ± SEM (n > 3). (*) P < 0.05. (B) MCF7 cells were transfected with phpR-egr2-F. Forty-eight hours after transfection, cells were treated for 4 h with CM or CM containing SB203580 (10 μM). IRES activity was calculated as described before and is given relative to CM. Data are presented as means ± SEM (n > 3). (**) P < 0.01.