The RTD phenotypes of single-modification mutants are exacerbated by a tef1-Δ mutation. (A) A tef1-Δ mutation exacerbates the temperature sensitivity of trm8-Δ mutants, tan1-Δ mutants, and trm1-Δ mutants. Strains were grown overnight in YPD media, serially 10-fold diluted, plated on YP plates containing 3% glycerol (YPG), and incubated for 4 d at the indicated temperatures. (B) A met22-Δ mutation fully suppresses the temperature sensitivity of trm8-Δ mutants and trm8-Δ tef1-Δ mutants. Strains were grown, diluted, and plated on YPD plates as described in Figure 3A. Similar results are observed in YPG media (data not shown). (C) A met22-Δ mutation fully suppresses the temperature sensitivity of tan1-Δ and trm1-Δ mutants, and their tef1-Δ–derivative strains. Strains were grown, diluted, and plated on YPD plates as described in Figure 3A. Similar results are observed in YPG media (data not shown).
