Lin28-mediated control of let-7 microRNA expression by alternative TUTases Zcchc11 (TUT4) and Zcchc6 (TUT7)

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FIGURE 4.
FIGURE 4.

Zcchc11 and Zcchc6 function redundantly to suppress let-7 expression in embryonic cells. (A) qRT–PCR analysis of mature let-7g and mature miR-21 levels in P19 embryonal carcinoma cells after transfection with the indicated siRNAs (left). mRNA levels of the indicated genes in P19 cells after transfection with the indicated siRNAs (right). (B) qRT–PCR as in A in V6.5 mouse embryonic stem cells. For all experiments, miRNA levels were normalized to sno-142 and mRNA levels were normalized to β-actin. Error bars represent SD of experiments in triplicate.

This Article

  1. Published in Advance August 16, 2012, doi: 10.1261/rna.034538.112 RNA 18: 1875-1885

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