Functional characterization of two paralogs that are novel RNA binding proteins influencing mitochondrial transcripts of Trypanosoma brucei

MRB8170 appears to preferentially bind mRNA in vitro. Binding curves of MRB8170 binding to (A) pre-edited RPS12 mRNA, (B) edited RPS12 mRNA, and (C) gA6[14] gRNA, which possesses a U₁₇ 3′-oligomer tail. The range of protein concentrations used is given on the x-axis in nanomolars and the fraction of bound RNA on the y-axis. The apparent K_d determined from the fitted curves is given along with the B_max of MRB8170 RNA binding and coefficient of determination (R²) of each curve. Whiskers at each data point represent standard deviation over two separate experiments done in triplicate.