Analysis of randomly selected 3′ sequences in the context of the “21-8p” modification pattern. (A) Activity of miR-124 single strands with randomized bases at positions 10 through 19. CD164 mRNA levels were measured by qRT-PCR following transfection of HCT-116 cells at 10 nM. (B) Sequence logo of miR-124 single strands with activity in the top quartile. (C) Pyrimidine-rich 3′ cassette can enhance single-strand miR-122 activity. Dose response curve of miR-122 target GYS1 to increasing amounts of the indicated oligos. (Red) miR-122 control duplex, (black) nontargeting control siRNA, (green) miR-122 sequence in the “21-8p” modification pattern, and (blue) miR-122 seed sequence fused with the “9Y_2” pyrimidine-rich 3′ cassette sequence, also in the 21-8p modification pattern.
