Single-stranded microRNA mimics

Restoration of activity by addition of 3′ and 5′ passenger strand fragments linked by carbon spacers. HCT-116 cells were transfected at 10 nM, 4 nM, and 2 nM, and CD164 mRNA was measured by qRT-PCR. 2′-OH is depicted in red, 2′-fluoro in green, 2′-o-methyl in black, LNA in light blue, 5′ phosphate in purple, and inverted abasic in yellow. The C3-spacer (ChemGenes CLP-9908) is represented by a dash.