Single-stranded microRNA mimics

Activity of the fluorinated and phosphorylated miR-124 single strand is enhanced by the addition of 5′ and 3′ passenger fragments, but either fragment alone decreases activity. Dose-dependent response of CD164 mRNA in HCT-116 cells, as measured by qRT-PCR. Schematic of annealed strands depicts modified strands and passenger segments, where 2′-OH is denoted in red, 2′-fluoro is green, 2′-o-methyl is black, and 5′ phosphate is purple.