FIGURE 2.
Microarray analysis shows seed-based activity from a miR-124 single strand. Microarray signature 24 h after transfection of 10 nM miR-124 21-8p into HCT116 cells, plotted as ratio of fluorescence intensity (relative to mock transfection) on the y-axis and mean fluorescence intensity for the experimental and mock samples on the x-axis. Significantly down-regulated probes (P < 1 × 106) are shown in green and significantly up-regulated probes in red. Hypergeometric analysis of the hexamer and heptamer content of the down-regulated 3′ UTRs showed that the most significantly enriched heptamers and hexamers are GTGCCTT and GTGCCT, respectively.
