VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus

Transcriptomic effects of VapBC6 deficiency. Relative transcript abundance of genes were determined by qRT–PCR in total RNA samples from the vapB6 disruption mutant and wild-type strain. (A) Transcripts whose abundance increased in the vapB6::lacS mutant relative to the wild type during heat shock. (B) Transcripts whose abundance decreased in the vapB6::lacS mutant relative to the wild type during heat shock. RNA levels were determined from cells growing exponentially at 80°C (filled bars), or 5 min after temperatures of the cultures reached 90°C (light gray bars), or 95°C (dark gray bars).