FIGURE 3.
Complementation of vapB6::lacS disruption using malA-based selection. (A) Complementation plasmid pBN1096. Plasmid pBN1096 was derived from plasmid pBN1080 by insertion of vapBC6 into the NotI site. Plasmid pBN1080 was derived from pBN1074 (Berkner et al. 2007) by replacment of lacS with malA at the SacII site. (B) Percent survival of the vapB6 disruption mutant and complemented strain during heat shock. Cultures were shifted to 95°C and samples were taken thereafter every 0.5 h. Strains were: wild-type (PBL2025, •); vapB6::lacS disruption (PBL2081, ○), vapB6::lacS complemented mutant (PBL2082, ▾).
