RT–PCR analysis of vapBC6. (A) Schematic of vapBC6 locus. Both vapB6 (SSO1494) and vapC6 (SSO1493) are transcribed from the antisense strand (leftward). Sites are shown for: the lacS disruption (inverted triangle); putative intragenic terminator; in frame deletions (heavy lines beneath boxed ORFs) and primers (horizontal arrows) that verify cotranscription. A 100-nt scale bar is indicated. (B) Cotranscription of vapB6 and vapC6. Detection of transcript complementary to the intergenic region used primers VapBC6RT-F, located 51 nt from the 3′ end of vapB6 and VapBC6RT-R located 51 nt from the 5′ end of vapC6. Samples: (lane 1) MW markers; (lane 2) complete reaction; (lane 3) no addition of reverse transcriptase; (lane 4) 7S RNA positive control using primers 7S-F and 7S-R.
