Adapter ligation to 5′-ends of RNA templates with different oligonucleotides. (A) SalI adapter ligation to 5′-ends of 5′-monophosphorylated RNAs. (B) BC1 RNA ligation with fusion SalI adapters; the SalI restriction site was exchanged for the Roche MID tags (MID1–12), the resulting linkers being Ad1–12. (C) Different RNA templates ligated to Ad1 adapter derivates. Either 3′-nucleotides or sugars were altered (Table 2). (A–C) Lanes 1 and 2 represent control reactions. (Lane 1) Ligase and adapter were not added. (Lane 2) No adapter was added. Values to the right represent molecular sizes. Potential RNA circularization depicted as in Figure 2. (A–C) Oligonucleotides' sequence and abbreviations across the top of the gels are defined in Table 2.
