Northern blot hybridization and statistical overview. (A) Graphical representation of candidate npcRNAs from Liu et al. (2009) (ultra-deep pyrosequencing) with those generated from our approach (conventional cDNA sequencing). (B) Examples of Northern blot hybridizations of npcRNA candidates that are shared in both data sets (Supplemental Table 3). (C) Northern blot hybridization analysis of npcRNA candidates found only in this study. (B,C) Northern blot analysis was performed on total RNA isolated from V. cholerae O1 El Tor clinical isolate VC3321 (VC3321) and V. cholerae O1 El Tor clinical isolate N16961 (N16961) at different OD600 values (Supplemental Fig. 1B), as shown at the top. npcRNA designations are indicated on the left and RNA sizes in nucleotides on the right.
