Substrate specificity and mutational analysis of Kluyveromyces lactis γ-toxin, a eukaryal tRNA anticodon nuclease

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FIGURE 4.
FIGURE 4.

Effect of an all-DNA stem on γ-toxin activity. The 39-mer oligonucleotide substrates are shown. The R39 substrate is all-RNA. D16•R7•D16 is a chimera in which the 16-bp stem is all-DNA and the anticodon loop is all-RNA. In D17•R2•D20 only the wobble U and the 5′ flanking U are ribonucleosides. Nuclease reaction mixtures contained 200 fmol 5′ 32P-labeled 30-mer substrates and γ-toxin as specified. The extents of 39-mer cleavage are plotted as a function of input γ-toxin.

This Article

  1. Published in Advance May 24, 2011, doi: 10.1261/rna.2722711 RNA 17: 1336-1343

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