Substrate specificity and mutational analysis of Kluyveromyces lactis γ-toxin, a eukaryal tRNA anticodon nuclease

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 3.
FIGURE 3.

Effect of 5′ or 3′ stem deoxynucleosides on γ-toxin activity. The 30-mer oligonucleotide substrates are shown. The R30 substrate is all-RNA. D16R14 is a chimera in which the proximal (5′) 16 nucleotides (nt) are deoxys and the distal (3′) 14 nt are ribos. D18R12 is composed of a proximal 18-nt DNA segment and a distal 12-nt RNA segment. R23D7 is composed of a proximal 23-nt RNA segment and a distal 7-nt DNA segment. Nuclease reaction mixtures contained 200 fmol 5′ 32P-labeled 30-mer substrates, and γ-toxin as specified. The extents of 30-mer cleavage are plotted as a function of input γ-toxin.

This Article

  1. Published in Advance May 24, 2011, doi: 10.1261/rna.2722711 RNA 17: 1336-1343

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA