Optimal nucleotide bias in (G/T)15 P2 oligo 3′-extensions. The polymerization efficiency is shown as a function of the percentage of guanosines in the 3′-extensions to the DNA P2 oligo sequence 5′-GGCGCC. Extensions with defined sequences (filled diamonds) or a randomized sequence with 50% G (open triangle) were investigated. The values for polymerization efficiency are the geometric means for polymerization efficiencies with the templates T21, T50a, T50b, and T50c. Standard deviations of triplicate experiments are shown as error bars but are often smaller than the symbols in the graph. The P2 oligo sequences and the values of polymerization efficiency are given in Supplemental Table S4.
