Effect of trinucleotide RNA P2 oligos on polymerization efficiency with four different templates. For template T21 (A), a representative autoradiogram of PAGE-separated polymerization products and its quantitation is shown. The concentrations of the trinucleotides (0 μM to 128 μM) and the number of added nucleotides (nts) are indicated on the autoradiogram. For the other templates, (B) template T50a, (C) template T50b, and (D) template T50c, only the quantitation is shown. The quantitations show a plot of the average number of nucleotides added per primer as a function of the concentration of the trinucleotides 5′-GGC (filled squares) or 5′-NNN (open squares). Standard deviations of triplicate experiments were smaller than the symbols in the graph, typically in the range of 0.03 nt/primer.
