Polymerase ribozyme efficiency increased by G/T-rich DNA oligonucleotides

Secondary structure of the polymerase ribozyme Pol1e. The secondary structure is based on Johnston et al. (2001), Shechner et al. (2009), and Wang et al. (2011). (Blue) The wild-type P2 oligo; (green) the primer/template substrate with the sequence of template T21 and the corresponding primer. (Arrowheads) Residues close to the catalytic site. These are the unpaired C in helix P4 (Shechner et al. 2009), the 3′-terminus of the P2 oligo (Ekland and Bartel 1996), and the loop connecting helices A3 and A4 (Wang et al. 2011). (Dotted lines) The tertiary contacts between the accessory domain and ligase core (Wang et al. 2011).