Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae

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FIGURE 5.
FIGURE 5.

In vitro reconstitution of m3C32 by recombinant Trm140p. (A) C-terminal hexahistidine-tagged recombinant Trm140p resolved on SDS-PAGE and stained with Coomassie brilliant blue (CBB). (B) Mass chromatograms detecting MH+ (m/z 258) of m3C (and m5C) in total nucleosides from individual tRNAThr1 (top left) and tRNASer1 (bottom left) isolated from the wild-type strain. (Right) The same mass chromatograms (m/z 258) of tRNAThr1 (top right) or tRNASer1 (bottom right) in the absence (−Ado-Met) or presence (+Ado-Met) of Ado-Met.

This Article

  1. Published in Advance April 25, 2011, doi: 10.1261/rna.2653411 RNA 17: 1111-1119

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