Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae

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FIGURE 4.
FIGURE 4.

Complementation of the yor239wΔ strain with plasmid-encoded ABP140 containing various mutations. LC/MS analysis of total nucleosides isolated from yor239wΔ strains with various ABP140 constructs containing various mutations; WT(0-frame), delN, D466A, F481A, D547A, K559A, Y569A, and del(D602-Q621). Mass chromatograms detecting MH+ (m/z 258) of m3C, m5C, and Cm are shown. Arrows indicate the retention time for m3C.

This Article

  1. Published in Advance April 25, 2011, doi: 10.1261/rna.2653411 RNA 17: 1111-1119

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