Domain structure and sequence alignment of the ABP140/TRM140 protein and its homologs. (A) Domain structure of the S. cerevisiae ABP140/TRM140 protein. Positions of YOR239w and YOR240w are shown. The actin-binding motif, +1 frameshift site, and Ado-Met binding motif are indicated. (B) Sequence alignment of ABP140 and its homologs from fungi and other eukaryotes. All of these homologs were previously indicated (Farabaugh et al. 2006). The conserved actin-binding site and Ado-Met binding motif are underlined. The 7-mer slippage sequence is indicated at the +1 frameshift site. Motifs I–VI of Rossmann-fold-type class I methyltransferases are lined at the top of the sequence. The residues and C-terminal conserved region mutated in this study are indicated. Accession numbers are NC_006033.1 (Candida glabrata), NP_983532 (Ashbya gossypii), CAB76043.1 (Schizosaccharomyces pombe), BAE31989 (Mus musculus), NP_060866 (Homo sapiens), and NP_647636 (Drosophila melanogaster).
