A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop

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FIGURE 5.
FIGURE 5.

ORF YOR239W is not required for m3C methyltransferase activity in vivo. A trm140-Δ strain was transformed with CEN plasmids containing TRM140-Δ12-276 (lane a), TRM140 (lane b), or an empty vector (lane c), and bulk RNA (2 μg) prepared from these strains was subjected to primer extension as described in Figure 2. (Lane d) No RNA.

This Article

  1. Published in Advance April 25, 2011, doi: 10.1261/rna.2652611 RNA 17: 1100-1110

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