Suppression of pre-miR-886 inhibits cell proliferation. (A) Three anti-oligos targeting pre-miR-886 (anti886 62_43, anti886 45_26, and anti886 75_56) and two control anti-oligos (anti886 mut62_43 and anti_vt 21_2). Their sequences (in bold letters) are aligned with pre-miR-886 or vtRNA1-1, with mismatched nucleotides highlighted in gray. Arrow indicates Northern probe used in panel B. (B) Northern hybridization of pre-miR-886 after transfection of indicated anti-oligos in HeLa and HCT116 cells. Cells were harvested for RNA preparation 24 h after transfection of each anti-oligo at 40 nM (also for panels C,D). (C,D) Cell proliferation assays (MTT assays) in indicated cell lines after transfecting indicated anti-oligos. Plain bars designate control anti-oligos or no oligo (mock transfection without oligonucleotides), and black bars designate the three targeting anti-oligos. Below each cell line, the relative expression level of pre-miR-886 is indicated (see also Fig. 1D and Supplemental Fig. S1).
