Discrimination of let-7 miRNA isoforms. (A) Sequences of selected members of let-7 miRNA family. The nucleotide differences between let-7a and the other isoforms are underlined. (B) Relative detection (in percent) of let-7 isoforms by miR-ID assays, in which every individual isoform was assayed with every isoform-specific primer pair. For each assay, the difference in Ct value for its intended isoform and the other isoforms was converted into a linear percentage, with the fully matched reaction (diagonal values) made equal to 100%. (Bold numbers) miR-ID; (numbers in parentheses) published values (other than 0.0) for QIAGEN's miScript/SYBR Green (*), ABI's TaqMan (**), and LNA/SYBR Green (***) miRNA RT-qPCR assays.
