miR-ID: A novel, circularization-based platform for detection of microRNAs

Sensitivity and detection limit of an individual miRNA are not affected by the presence of total cellular RNA. Synthetic miR-127 miRNA having 5′-p and 2′-OH/3′-OH ends was used in this example. Standard curves were generated for a dilution series from 200 pM to 200 aM miR-127 for miR-ID assays performed either in the absence (squares) or the presence of 20 ng of total cellular RNA (diamonds). The total RNA was extracted from Jurkat cells, which do not express miR-127.