miR-ID: A novel, circularization-based platform for detection of microRNAs

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FIGURE 4.
FIGURE 4.

Reverse transcription of a circular miRNA template (RTC). Circularized synthetic miR-127 miRNA (80 nM) was used as template for SSII reverse transcriptase with 10- and 19-nt RT primers (1 μM) in the presence of [α-32P]dATP. The resulting 32P-labeled primer-extension products were analyzed on a denaturing 12% polyacrylamide gel. (Lane L) A 5′-32P-labeled 10-bp DNA ladder. (Lane 1) Linear 5′-32P-labeled miR-127 as a control. (Lanes 2,3) The extension products of 19- and 10-nt primers, respectively. (Lane 4) A negative control in which the RTC reaction with the 10-nt primer was carried out without the miRNA template.

This Article

  1. Published in Advance December 17, 2010, doi: 10.1261/rna.2490111 RNA 17: 365-380

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