FIGURE 5.
Isolation of proteins binding to a transcript derived from exon 4 of the CD45 exonic splicing silencer—ESS1 or a mutant version—ESS1mt. (A) Sequences of ESS1 and ESS1mt RNAs are shown. Mutated residues are in bold. (B) Standard and (C) RNase-assisted chromatography of ESS1 and ESS1mt transcripts in HeLa nuclear extracts. Bands denoted A, B, C, D, and E correspond to selected proteins highly enriched in the ESS1 chromatography, hnRNP K, hnRNP L, FUBP2, FUBP1, and FUBP3, respectively. (Bottom) hnRNP L Western blot analysis of the corresponding RNA chromatograms. (*) A band corresponding to RNase A/T1.
