Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I

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FIGURE 6.
FIGURE 6.

The PRMT5/WD45 complex is needed for methylation of the CF Im68 GAR motif. The control substrate Sm proteins D1/D2 (1 μg) and GST-68_GR (800 ng) were incubated in standard methylation reactions with 1 μL nuclear extract (NE) or cytoplasmic extract (CE) or with increasing amounts of the PRMT5 complexes as indicated. Amounts of enzyme present in the reactions were (from left to right) PRMT5/pICln: 10, 100, and 1000 ng; PRMT5/WD45 and PRMT5/WD45/pICln: 1, 10, and 100 ng.

This Article

  1. Published in Advance June 18, 2010, doi: 10.1261/rna.2164210 RNA 16: 1646-1659

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