Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I

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FIGURE 5.
FIGURE 5.

Fractionation of the HeLa cytoplasmic extract on the HiTrap DEAE (A), HiTrap heparin (B), and Superdex-200 (C) gel filtration columns. Fractions were probed on Western blots with antibodies against PRMT5, WD45, pICln, and PRMT1 and an activity assay was performed with substrates GST-68_GR and [3H]SAM. L, load; P, pellet; FT, flow through; and PWI indicates 900 ng of the PRMT5/WD45/pICln complex. The numbers are the column fraction numbers.

This Article

  1. Published in Advance June 18, 2010, doi: 10.1261/rna.2164210 RNA 16: 1646-1659

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