Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I

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FIGURE 4.
FIGURE 4.

Activity of PRMT1-deficient cell extracts on protein substrates. Cell extracts from PRMT1(+/+) and PRMT1(−/−) ES cells were incubated with [14C]SAM and recombinant GST-68_GRP, PABPN1, or hnRNP K (10 pmol each). Reactions were resolved on 12% SDS PAGE and methylation was detected by autoradiography.

This Article

  1. Published in Advance June 18, 2010, doi: 10.1261/rna.2164210 RNA 16: 1646-1659

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